Using Histological Staining Techniques to Improve Visualization and Interpretability of Tooth Cementum Annulation Analysis

The aim of the present study was to evaluate effect different staining techniques on applicability and accuracy tooth cementum annulation (TCA) method. Nine decalcination techniques, 8 dehydration protocols were applied in 3 teeth from persons a known age. Black white, color images histological sections captured. An x- ray taken each they photographed. Researchers asked observe both black/white sections. divided into two groups. first group analyzed only, second had photos X-rays. In observers (without X ray) differences age estimation between real observed significant for 2 younger patients, but not oldest patient, where values matched. Of 6 raters, assesments last (that used x-ray together with sections) did differ significantly values. Extensive analysis multiple repetitions performed investigation revealed that most optimal method decalcification TCA EDTA II period longer than 14 days at section thickness 2-3mm, while protocol number IV. When it comes staining, cemental lines visualization counting Crocein Scarlet/Acid Fuchsin Toluidine blue semithin section. Additional use preexperimental evaluation employing decreased errors estimation.